《植物生理学报》 2014, 50(6): 875-879

晚熟脐橙果实发育过程中抗坏血酸含量及相关酶活性的变化

黄艳花^1,2, 曾明^1,* , 王玲利^1,2, 苏芳芳^1,2, 李兴发^1,2

¹西南大学园艺园林学院, 重庆400716; ²南方山地园艺学教育部重点实验室, 重庆400716

通信作者：李兴发；E-mail: zengming@swu.edu.cn；Tel: 023-68251158

摘　要：

以‘鲍威尔’脐橙为试材, 研究了果实发育期间果皮和果肉中抗坏血酸(AsA)含量及相关酶活性的变化。结果表明, 果皮中总抗坏血酸(T-AsA)和AsA含量显著高于果肉, 且在发育期间T-AsA和AsA的变化趋势一致; 果皮中L-半乳糖内酯脱氢酶(GalLDH)活性与T-AsA和AsA积累速率的变化趋势基本一致, 呈显著正相关关系, 而在果肉中的变化趋势却不明显; 在发育过程各阶段中果皮的抗坏血酸氧化酶(AAO)、抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)和单脱氢抗坏血酸还原酶(MDHAR)活性均高于果肉; 相关性分析显示, 果皮中AsA含量积累主要取决于GalLDH活性, 而果肉中AsA含量水平可能取决于AsA的再生循环系统。

关键词：‘鲍威尔’脐橙; 果皮; 果肉; 抗坏血酸; 酶活性

收稿：2014-01-16   修定：2014-05-16

Changes in Ascorbic Acid Contents and Related Enzyme Activities during Fruit Development of Late-Maturing Navel Orange

HUANG Yan-Hua^1,2, ZENG Ming^1,* , WANG Ling-Li^1,2, SU Fang-Fang^1,2, LI Xing-Fa^1,2

¹School of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China; ²Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400716, China

Corresponding author: ZENG Ming; E-mail: zengming@swu.edu.cn; Tel: 023-68251158

Abstract:

In this research, ascorbic acid (AsA) content and enzyme activities in peel and pulp of navel orange (Citrus sinensis cv. ‘Powell’) were investigated during fruit development. The results showed that total AsA (T-AsA) and AsA contents in peel were higher than those in pulp, the accumulation of T-AsA and AsA during fruit development showed a similar tendency. In peel, the variation of L-galactono-1,4-lactone dehydrogenase (GalLDH) activity coincided with the accumulation rates of T-AsA and AsA, and presented significant positive correlation between the GalLDH activity and AsA accumulation rate, while in pulp, GalLDH activity almost kept constantly during fruit development. The activities of ascorbic acid oxidase (AAO), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) in the peel were higher than those in the pulp during fruit development. Correlation analysis indicated that the AsA concentration in the peel was mainly determined by GalLDH activity, but in the pulp the AsA content may depend on the regulation of AsA regeneration capacity.

Key words: ‘Powell’ navel orange; peel; pulp; ascorbic acid; enzyme activity